Nucleotide sequence of a new YPT1-related human cDNA which belongs to the ras gene superfamily.

We first found a partial coding region of a new YPT1-related human gene in an aberrant cDNA clone fused with an anti-sense C_ region transcript of the immunoglobulin K gene, isolated from a Burkitt lymphoma cell line KOBK101 (1) carrying a t(2;8) translocation. Then, the complete cDNA clone was isolated from a human promonocytic cell line U-937 (2). The nucleotide and the deduced amino acid sequences of the new YPT1-related human cDNA, designated as Hrab2 and shown below, have homologies of 92.9% and 98.11 with those of a YPT1-related rat cDNA rab2 (3), respectively. The polypeptide coded by Hrab2 is calculated to be about 23,500 daltons and conserves the four GTP-binding regions common to the ras proteins (shown by underlines).